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KMID : 0043320110340091495
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Archives of Pharmacal Research
2011 Volume.34 No. 9 p.1495 ~ p.1501
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Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells
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Jin Meihua
Lu Yue
Yang Ju-Hye
Jo Tae-Hyung
Park Young-In
Lee Chong-Kil
Park Sang-Jo
Son Kun-Ho
Chang Hyeun-Wook
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Abstract
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6-Hydroxy-2,7-dimethoxy-1,4-phenanthraquinone (PAQ) isolated from the tuberous roots of Yam (Dioscorea batatas) inhibited cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) dependent prostaglandin D2 (PGD2) generation in mouse bone marrow-derived mast cells in a concentration-dependent manner with IC50 values of 0.08 μM and 0.27 μM, respectively. In the Western blotting with specific anti-COX-2 antibodies, the decrease of the quantity of PGD2 was accompanied by a decrease in the COX-2 protein level. But PAQ did not affect COX-1 protein level. In addition, this compound inhibited 5-lipoxygenase (5-LOX) dependent production of leukotriene C4 in a dose-dependent manner, with an IC50 of 0.032 μM. These results demonstrate that PAQ has a dual COX-2/5-LOX inhibitory activity. This compound also inhibited the degranulation reaction in a dose-dependent manner with an IC50 of 2.7 μM. Thus, these results suggest that PAQ may be useful in regulating mast cell-mediated inflammatory diseases.
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KEYWORD
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Yam, 6-Hydroxy-2, 7-dimethoxy-1, 4-phenanthraquinone, Cyclooxygenase-2, 5-lipoxygenase, Bone marrow-derived mast cells
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